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Expression of glucose-regulated protein 78 as prognostic biomarker for triple-negative breast cancer

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dc.contributor.authorYang, Chenlian
dc.contributor.authorZhang, Zhiwei
dc.contributor.authorZou, Yutian
dc.contributor.authorGao, Guanfeng
dc.contributor.authorLiu, Lingrui
dc.contributor.authorXu, Haifan
dc.contributor.authorLiu, Feng
dc.date.accessioned2022-11-22T11:33:14Z
dc.date.available2022-11-22T11:33:14Z
dc.date.issued2020
dc.description.abstractIntroduction. glucose-regulated protein78 (GRP78) is a stress - induced endoplasmic reticulum chaperone protein. It is closely related to the occurrence, development, proliferation, differentiation and drug resistance of breast cancer. However, the association and clinicopathological features between GRP78 and triple negative breast cancer (TNBC) remain to be studied. Material and Methods. Clinical and pathological characteristics and overall survival were analysed retrospectively in 179 surgically resected TNBC patients. GRP78 was detected by immunohistochemistry (IHC) using breast cancer tissue microarrays (TMAs), and the association between GRP78 levels and clinicopathological factors and prognosis was analyzed. Furthermore, GRP78 expression in human TNBC and NTNBC cell lines was detected by Western blot and qRT-PCR. After Si-GRP78 knocked-down GRP78 in MDA-MB-231 and BT549 cell lines, cell proliferation was detected using Cell Counting Kit-8 (CCK-8) and cell colony formation was detected by crystal violet staining, respectively. Results. GRP78 was expressed in triple negative breast cancer (TNBC). GRP78 expression was significantly associated with invasive, distant metastasis and proliferation of TNBC (P<0.05). In addition, patients with positive GRP78 expression had shorter overall survival (OS) and disease-free survival (DFS). And the high expression of GRP78 was significantly associated with disease-free survival (DFS) in patients with TNBC (P<0.001). Conclusions. These findings improve our understanding of the expression pattern of GRP78 in TNBC and clarify the role of GRP78 as a promising prognostic biomarker for triple-negative breast cancer.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology Vol. 35, nº6 (2020)
dc.identifier.doihttps://doi.org/10.14670/HH-18-185
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/125824
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectGRP78es
dc.subjectProliferationes
dc.subjectPrognosises
dc.subjectTriple negative breast canceres
dc.subjectMetastasises
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleExpression of glucose-regulated protein 78 as prognostic biomarker for triple-negative breast canceres
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.35, nº6 (2020)