Publication: Degree of correspondence between contractile and oxidative capacities in horse muscle fibre, a histochemical study
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Date
1990
Authors
López-Rivero, J.L. ; Agüera, E. ; Rodríguez Barbudo, M. V. ; Galisteo, A. M. ; Morales-López, J. L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Samples taken from the middle gluteal
muscle of 95 untrained adult horses of different ages and
sex were subjected to histochemical analysis using the
myosin adenosine triphosphatase (m-ATPase) and
nicotinamide adenine dinucleotide tetrazolium reductase
(NADH-TR) staining techniques. Fibres were classified
into types 1, IIA and IIB according to m-ATPase activity
after preincubation at pH 4.4. The percentage of FT
(Fast-Twitch Glycolytic) fibres and the proportion of IIB
fibres with «high» and «low» oxidative capacity were
determined in serial sections stained for NADH-TR.
Statistical analysis revealed a significantly higher
proportion of IIR fibres than FT fibres (P < 0.001),
though both percentages were correlated. Thus, 72.2
+/- 17.6% of type IIB fibres showed low oxidative
capacity , but the remaining 27.8 +/- 17.6% showed high
aerobic potential, and thus did not correspond to FT
fibres.
These results confirm that the contractile capacity of a
muscle fibre does not determine its oxidative profile. The
different types of muscle fibre should thus be classified
solely according to m-ATPase activity, since this
characteristic is related to the molecular structure of
contractile proteins. Oxidative capacity should be
assessed separately, and not be used as a criterion for
fibre classification in horses.
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