Publication: Empoasca papayae Oman, 1937 (Hemiptera: Cicadellidae) the simultaneous vector of phytoplasmas and rickettsia associated with “Bunchy Top Symptom” in Cuba
Authors
Acosta, Karel Ismar ; Zamora, Loidy ; Piñol, Berta ; Quiñones, Madelaine Luisa ; Ramos, Pedro Luis ; Luis, Maritza ; Leyva-López, Norma Elena ; Arocha, Yaima
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Publisher
Universidad de Murcia
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DOI
http://dx.doi.org/10.6018/analesbio.39.03
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info:eu-repo/semantics/article
Description
Abstract
Ensayos de transmisión mostraron desarrollo de síntomas típicos
de BTS en todas las plantas de papaya después de tres meses de
post-inoculadas con Empoasca papayae. El fitoplasma identificado
en 28 de 45 las plantas inoculadas, presentó, en secuencia de
ADN ribosomal 16S, un 99,9% igual al identificado en plantas de
campo y con las detectadas previamente en E. papayae en Cuba.
Todos son miembros del subgrupo de fitoplasma 16SrI-B. Una rickettsia
se identificó en 41 de las 45 plantas inoculadas. Su secuencia
parcial del gen sdhA mostró un 100% de identidad con la
rickettsia identificada en plantas de campo, E. papayae y las previamente
publicadas. Los resultados confirmaron la habilidad de E.
papayae para transmitir simultáneamente el fitoplasma y la rickettsia.
Abstract: Transmission trials showed typical BTS symptoms developed in all papaya plants after three months post-inoculated with Empoasca papayae. The BTS phytoplasma was identified in 28 out of 45 inoculated papayas, whose partial 16S rDNA sequence was 99.9% identical to those of the phytoplasma identified in the field-collected papayas, and that previously detected in E. papayae in Cuba. These are all members of the phytoplasma subgroup 16SrI-B. A rickettsia was identified in 41 out of 45 inoculated papayas. It showed a 100% of identity of its partial sdhA sequence to those from the field-collected papayas, E. papayae and the rickettsia previously identified. Results confirmed the ability of E. papayae to simultaneously transmit the BTS phytoplasma and the rickettsia pathogen.
Abstract: Transmission trials showed typical BTS symptoms developed in all papaya plants after three months post-inoculated with Empoasca papayae. The BTS phytoplasma was identified in 28 out of 45 inoculated papayas, whose partial 16S rDNA sequence was 99.9% identical to those of the phytoplasma identified in the field-collected papayas, and that previously detected in E. papayae in Cuba. These are all members of the phytoplasma subgroup 16SrI-B. A rickettsia was identified in 41 out of 45 inoculated papayas. It showed a 100% of identity of its partial sdhA sequence to those from the field-collected papayas, E. papayae and the rickettsia previously identified. Results confirmed the ability of E. papayae to simultaneously transmit the BTS phytoplasma and the rickettsia pathogen.
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Citation
Anales de Biología, nº 39, (2017)
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