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Rabbit zona pellucida composition: A molecular, proteomic and phylogenetic approach

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dc.contributor.authorStetson, I.
dc.contributor.authorChevret, P.
dc.contributor.authorLorenzo, P.L.
dc.contributor.authorBallesta, J.
dc.contributor.authorRebollar, P.G.
dc.contributor.authorGutiérrez-Gallego, R.
dc.contributor.authorIzquierdo Rico, María José
dc.contributor.authorAvilés Sánchez, Manuel
dc.contributor.authorMoros Nicolás, Carla
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2024-01-22T13:27:22Z
dc.date.available2024-01-22T13:27:22Z
dc.date.issued2012
dc.description©2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Proteomics. To access the final edited and published work see https://doi.org/10.1016/j.jprot.2012.07.027es
dc.description.abstractThe zona pellucida (ZP) participates in sperm–egg interactions during the first steps of 22 fertilization. Recent studies have shown that the ZP matrix of oocytes in several species is 23 composed of four glycoproteins, designated as ZP1, ZP2, ZP3 and ZP4, rather than the three 24 described in mouse, pig and cow. In this study, investigations were carried out to unveil a Q425 fourth glycoprotein in the rabbit (Oryctolagus cuniculus) ZP. Using total RNA isolated from 26 rabbit ovaries, the complementary deoxyribonucleic acid (cDNA) encoding rabbit ZP1 was 27 amplified by reverse transcribed polymerase chain reaction (RT-PCR). The ZP1 cDNA 28 contains an open reading frame of 1825 nucleotides encoding a polypeptide of 608 amino 29 acid residues. The deduced amino acid sequence of rabbit ZP1 showed high identity with 30 other species: 70% identity with human and horse ZP1, and 67% identity with mouse and rat 31 ZP1. At the proteomic level, peptides corresponding to the four proteins were detected by 32 mass spectrometry. In addition, a molecular phylogenetic analysis of ZP1 showed that 33 pseudogenization of this gene has occurred at least four times during the evolution of 34 mammals. The data presented in this manuscript provide evidence, for the first time, that 35 the rabbit ZP is composed of four glycoproteins.es
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dc.format.extent16es
dc.identifier.citationProteomics
dc.identifier.doihttps://doi.org/10.1016/j.jprot.2012.07.027
dc.identifier.issn1615-9861
dc.identifier.issn1615-9853
dc.identifier.urihttp://hdl.handle.net/10201/137541
dc.languageenges
dc.relationFundación Seneca (04542/GERM/06), MICINN (AGL2009-12512-C02-01-02), MICINNAGL08-2283.es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectZona pellucidaes
dc.subjectRabbites
dc.subjectOocytees
dc.subjectFertilizationes
dc.subjectSperm–egg interactiones
dc.titleRabbit zona pellucida composition: A molecular, proteomic and phylogenetic approaches
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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relation.isAuthorOfPublicationeb2d72d3-a1d5-48cc-b71f-4aaf60a9aeac
relation.isAuthorOfPublication3acee413-7e4c-4025-90cb-5a0d9cb10014
relation.isAuthorOfPublication.latestForDiscoveryb0205f82-a44c-47b6-8061-b55efb4e6717
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