Publication: Rabbit zona pellucida composition: A molecular, proteomic and phylogenetic approach
Authors
Stetson, I. ; Chevret, P. ; Lorenzo, P.L. ; Ballesta, J. ; Rebollar, P.G. ; Gutiérrez-Gallego, R. ; Izquierdo Rico, María José ; Avilés Sánchez, Manuel ; Moros Nicolás, Carla
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DOI
https://doi.org/10.1016/j.jprot.2012.07.027
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info:eu-repo/semantics/article
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©2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted version of a Published Work that appeared in final form in Proteomics. To access the final edited and published work see https://doi.org/10.1016/j.jprot.2012.07.027
Abstract
The zona pellucida (ZP) participates in sperm–egg interactions during the first steps of
22 fertilization. Recent studies have shown that the ZP matrix of oocytes in several species is
23 composed of four glycoproteins, designated as ZP1, ZP2, ZP3 and ZP4, rather than the three
24 described in mouse, pig and cow. In this study, investigations were carried out to unveil a
Q425 fourth glycoprotein in the rabbit (Oryctolagus cuniculus) ZP. Using total RNA isolated from
26 rabbit ovaries, the complementary deoxyribonucleic acid (cDNA) encoding rabbit ZP1 was
27 amplified by reverse transcribed polymerase chain reaction (RT-PCR). The ZP1 cDNA
28 contains an open reading frame of 1825 nucleotides encoding a polypeptide of 608 amino
29 acid residues. The deduced amino acid sequence of rabbit ZP1 showed high identity with
30 other species: 70% identity with human and horse ZP1, and 67% identity with mouse and rat
31 ZP1. At the proteomic level, peptides corresponding to the four proteins were detected by
32 mass spectrometry. In addition, a molecular phylogenetic analysis of ZP1 showed that
33 pseudogenization of this gene has occurred at least four times during the evolution of
34 mammals. The data presented in this manuscript provide evidence, for the first time, that
35 the rabbit ZP is composed of four glycoproteins.
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Proteomics
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