Publication:
The presence of the u-opioid receptor in the isthmus of mare oviduct

Simple item page
dc.contributor.authorDesantis, S.es
dc.contributor.authorAlbrizio, M.es
dc.contributor.authorVentriglia, G.
dc.contributor.authorDeflorio, M.
dc.contributor.authorGuaricci, A.C.
dc.contributor.authorMinoia, R.
dc.contributor.authorDe Metrio, G.
dc.date.accessioned2013-01-22T10:01:02Z
dc.date.available2013-01-22T10:01:02Z
dc.date.issued2008
dc.description.abstractThe presence of the μ-opioid receptor and the type of glycosylation in the third extra-cellular loop of this receptor was investigated in the isthmus of mare oviduct during oestrus by means of immunoblotting and immunohistochemistry combined with enzymatic (Nglycosidase F and O-glycosidase) and chemical (ßelimination) treatments. Immunoblotting analysis showed that the μ -opioid receptor consists of two peptides with molecular weights of around 65 and 50 kDa. After N-deglycosylation with N-glycosidase F an additional immunoreactive peptide was observed at around 30 KDa. The cleavage of O-glycans by Oglycosidase failed in immunoblotting as well as in immunohistochemistry investigations, revealing that the third extra-cellular loop of the μ -opioid receptor expressed in mare isthmus oviduct contains some modifications of the Galß(1-3)GalNAc core binding to serine or threonine. Immunohistochemistry revealed the μ-opioid receptor in the mucosal epithelium, some stromal cells, muscle cells and blood vessels. In ciliated cells the μ-opioid receptor showed N-linked glycans, since the immunoreactivity was abolished after Nglycosidase F treatment, whereas it was preserved in the apical region after ß-elimination. Most non-ciliated cells expressed the μ-opioid receptor with both N- and Olinked oligosaccharides, as revealed by the abolition of immunostaining after N-glycosidase F and ßelimination. Stromal cells, endothelial and muscle cells of blood vessels expressed the μ -opioid receptor containing both N- and O-linked oligosaccharides. Myosalpinx myocytes expressed the μ-opioid receptor with O-linked oligosaccharides. The immunopositive myocytes formed a circular coat in the intrinsic musculature, whereas they were arranged in some isolated, oblique bundles in the extrinsic musculature. In conclusion, the μ-opioid receptor could have a role in the production and the movement of isthmus lumen content that contributes to ensuring the effective condition of the sperm in the mare oviduct.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/29799
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectOviductes
dc.subjectGlycosylationes
dc.subject.other636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleThe presence of the u-opioid receptor in the isthmus of mare oviductes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
The presence of the u-opioid receptor in the isthmus of mare oviduct.pdf
Size:
4.33 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.23, nº5 (2008)