Publication: The presence of the u-opioid receptor in the isthmus of mare oviduct
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Date
2008
Authors
Desantis, S. ; Albrizio, M. ; Ventriglia, G. ; Deflorio, M. ; Guaricci, A.C. ; Minoia, R. ; De Metrio, G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The presence of the μ-opioid receptor and the
type of glycosylation in the third extra-cellular loop of
this receptor was investigated in the isthmus of mare
oviduct during oestrus by means of immunoblotting and
immunohistochemistry combined with enzymatic (Nglycosidase
F and O-glycosidase) and chemical (ßelimination)
treatments. Immunoblotting analysis
showed that the μ -opioid receptor consists of two
peptides with molecular weights of around 65 and 50
kDa. After N-deglycosylation with N-glycosidase F an
additional immunoreactive peptide was observed at
around 30 KDa. The cleavage of O-glycans by Oglycosidase
failed in immunoblotting as well as in
immunohistochemistry investigations, revealing that the
third extra-cellular loop of the μ -opioid receptor
expressed in mare isthmus oviduct contains some
modifications of the Galß(1-3)GalNAc core binding to
serine or threonine. Immunohistochemistry revealed the
μ-opioid receptor in the mucosal epithelium, some
stromal cells, muscle cells and blood vessels. In ciliated
cells the μ-opioid receptor showed N-linked glycans,
since the immunoreactivity was abolished after Nglycosidase F treatment, whereas it was preserved in the
apical region after ß-elimination. Most non-ciliated cells
expressed the μ-opioid receptor with both N- and Olinked
oligosaccharides, as revealed by the abolition of
immunostaining after N-glycosidase F and ßelimination.
Stromal cells, endothelial and muscle cells
of blood vessels expressed the μ -opioid receptor
containing both N- and O-linked oligosaccharides.
Myosalpinx myocytes expressed the μ-opioid receptor
with O-linked oligosaccharides. The immunopositive
myocytes formed a circular coat in the intrinsic
musculature, whereas they were arranged in some
isolated, oblique bundles in the extrinsic musculature. In
conclusion, the μ-opioid receptor could have a role in the production and the movement of isthmus lumen
content that contributes to ensuring the effective
condition of the sperm in the mare oviduct.
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