Publication:
Usefulness of Genotype MTBDRplus assay in acid-fast bacilli positive smear specimens in Almeria, Spain

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48515248Z_2014_Usefulness of Genotype MTBDRplus assay in AFB smear positive specimens in southeastern Spain. .pdf(306.64 KB)
Date
2014-05-05
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Authors
Martínez-Lirola, Miguel José ; Muñoz-Dávila, María José ; García-de Viedma, Darío ; Cabezas Fernández, Teresa ; Luzón García, Pilar ; Indal-Tb Group
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Publisher
Elsevier Doyma
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Genética y Microbiología
DOI
http://dx.doi.org/10.1016/j.eimc.2014.02.017
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Description
© 2013 Elsevier España.___ This document is the published version of a published work that appeared in final form in Enfermedades infecciosas y microbiologia clinica (English ed.)___ To access the final edited and published work see: http://dx.doi.org/10.1016/j.eimc.2014.02.017
Abstract
tIntroduction: The urgent need for operational research evaluating test performance in a real-world settinghas been highlighted. The purpose of this study was therefore to evaluate the performance of MTBDRplusassay.Materials: According to the reference method, of the 155 clinical specimens with valid results, 147 weresusceptible to rifampicin (RIF) and isoniazid (INH), with 4 being multi-drug resistant (MDR) and 4 withisolated resistance to isoniazid (INH).Results: The results of the MTBDRplus assay were 100% concordant for the MDR and mono-resistantto INH specimens. However, the MTBDRplus assay showed a resistance pattern to RIF in one specimenwhich was classified as susceptible by the reference method. The majority of the specimens (118/75.6%)were also tested using the MTBDRplus method after culture on Lowenstein-Jensen media, showing 100%agreement with the results of the test directly from the specimens. An MTBDRplus test result was availablewithin an average of 8 days.Conclusions: Overall, MTBDR results showed excellent results when compared with the reference methodand achieved a significant time-reduction.
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Molecular susceptibility , Comparison , Reducción tiempo de respuesta , Genotype MTBDRplus
Citation
Enfermedades infecciosas y microbiologia clinica (English ed.) 2014 32(8):511–514
URI
https://hdl.handle.net/10201/158769
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