Publication: Ultrastructural analysis of HNK-1 + cells in human
peripheral blood and lymph nodes
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Date
1989
Authors
Yuda, Fumiaki ; Terashima, Kazuo ; Dobashi, Michio ; lshikawa, Masakazu ; lmai, Yutaka
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
HNK-1 positive (HNK-1+) cells in human
peripheral blood and lymph nodes were comparatively
analysed by means of immunohistochemistry and immunoelectron
microscopy. In peripheral blood, the HNK-1'
cells were grouped into large granular lymphocytes (LGLs),
small lymphocytes and intermediate forms, al1 of which had
many fine cytoplasmic processes. Except for smoothsurfaced
lymphocytes, they could not be distinguished from
helperlinducer T (OKT41Leu3a) cells and suppresssor/cytotoxic
T (OKT81Leu2a) cells. In double staining, HNKlfT3-
cells and HNK-1+T3+ cells could not be clearly
distinguished in terms of morphology, although the
former contained many LGLs. The HNK-1+ cells in the
lymph nodes accumulated in the light zones of the
germinal centers (GCs). These cells were small to
medium-sized lymphocytes with few electron-dense
granules and exclusively co-expressed helperlinducer T
cell antigens (HNK-1+T4+). Their cytoplasmic
projections were interwoven with those of the follicular
dendritic cells which trap immune complexes for a long
duration. These configurations suggesl that HNK-1+T4+
cells in GCs are engaged in an immunological regulation
of germinal center cells. On the other hand. large blastic
HNK-1+ cells were scattered outside the GCs and some of
them were in the process of mitosis. Furthermore, HNKl+
LGL-like cells with a few large electron-dense granules
were rarely seen. These observations indicate that the
HNK-1+ cells in the lymph nodes may proliferate outside
GCs and differentiate into LGLs with a strong natural
killer function.
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