Publication: Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd
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Date
2007
Authors
Alvarado, N.E. ; Cancio, I. ; Hylland, K. ; Marigómez, I. ; Soto, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Metallothioneins (MT) were localized by
immunochemistry in different organs and cell
compartments of turbot exposed to sublethal
concentrations (100 ppb) of Cd for 7 days. The
polyclonal rabbit anti-cod MT antibody (NIVA, Norway)
applied herein exhibited positive cross-reactivity with
turbot MTs. Immunoreactive MTs were localized in the
branchial epithelium, in the liver and in the kidney of
turbot. In Cd exposed fishes MTs were demonstrated
mainly in branchial chloride cells (CC) and to a lesser
extend in the area where progenitor cells are located and
in the cells of the respiratory epithelium (secondary
lamellae). A higher staining intensity for MTs was
observed in CC of the interlamelar space of the main
branchial epithelium in comparison with control CC.
MT-staining was also observed in the chondroblasts of
the cartilage and in the erythrocytes within blood vessels
both in control and Cd-exposed specimens. MT
immunoreaction was high in the liver hepatocytes and
weak in the epithelium of the proximal portion of the
kidney in exposed turbot. The tegument, spleen and
muscle were devoid of any immunolabelling in both
treatments. Ultrastructural studies at the transmission
electron microscope revealed that Cd-induced MTs were
mainly located in the cytoplasm of gill CC, the
lysosomes and the cytoplasm of hepatocytes and in the
basal labyrinth of kidney proximal nephrocytes. The
differential localization/induction of MTs in different cell types described hereby suggests that the
quantification of the specific expression of MT may be
used in biomonitoring programs as a biomarker of Cd
exposure in aquatic environments.
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