Publication: C-reactive protein measurements in meat juice of pigs
Authors
Gutiérrez, A.M. ; Martínez Subiela, Silvia ; Montes, A. ; Parra Muñoz, María Dolores ; Cerón Madrigal, José Joaquín
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.vetimm.2008.01.002
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Description
© 2008 Elsevier B.V. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Veterinary Immunology and Immunopathology. To access the final edited and published work see https://doi.org/10.1016/j.vetimm.2008.01.002
Abstract
A time-resolved immunofluorometric assay was evaluated for measurement of C-reactive protein in meat juice from diaphragmatic muscle collected from slaughtered pigs. Analytical and clinical validation of the method was performed by using meat juice samples, obtained by freezing and thawing muscle pieces. The intra- and inter-assay coefficients of variation ranged from 2.2–5.8% to 7.9–14.3%, respectively. The limit of detection was 0.00038 mg/ml. The method measured the CRP concentrations in a linear manner with a good accuracy (r = 0.99). CRP concentrations in serum were highly correlated with those in diaphragmatic meat juice (r = 0.90; p < 0.001). CRP concentrations were significantly higher in clinically affected pigs compared to non-diseased
pigs. The assay described here provides a sensitive method for measuring CRP concentrations in meat juice, which can represent a suitable alternative to serum or blood samples and simplifies the process of sampling collection at slaughter.
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Citation
Veterinary Immunology and Immunopathology 122 (2008) 250–255
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