Browsing by Subject "TR-IFMA"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    Embargo
    C-reactive protein measurements in meat juice of pigs
    (Elsevier, 2008-04-15) Gutiérrez, A.M.; Martínez Subiela, Silvia; Montes, A.; Parra Muñoz, María Dolores; Cerón Madrigal, José Joaquín; Ciencias Sociosanitarias
    A time-resolved immunofluorometric assay was evaluated for measurement of C-reactive protein in meat juice from diaphragmatic muscle collected from slaughtered pigs. Analytical and clinical validation of the method was performed by using meat juice samples, obtained by freezing and thawing muscle pieces. The intra- and inter-assay coefficients of variation ranged from 2.2–5.8% to 7.9–14.3%, respectively. The limit of detection was 0.00038 mg/ml. The method measured the CRP concentrations in a linear manner with a good accuracy (r = 0.99). CRP concentrations in serum were highly correlated with those in diaphragmatic meat juice (r = 0.90; p < 0.001). CRP concentrations were significantly higher in clinically affected pigs compared to non-diseased pigs. The assay described here provides a sensitive method for measuring CRP concentrations in meat juice, which can represent a suitable alternative to serum or blood samples and simplifies the process of sampling collection at slaughter.
  • Repository logo
    Publication
    Open Access
    Data for compounds included in the article: "A sensitive immunoassay for the quantitation of Pig-MAP in pig saliva samples"
    (2024-10-05) Piñeiro, M; Matas-Quintanilla, M; Miralles, A; Gutiérrez, AM; Medicina y Cirugía Animal