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Analysis of wt1a reporter line expression levels during proepicardium formation in the zebrafish

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dc.contributor.authorAndrés Delgado, Laura
dc.contributor.authorGalardi Castilla, María
dc.contributor.authorMercader, Nadia
dc.contributor.authorSantamaría, Luis
dc.date.accessioned2022-12-07T15:27:42Z
dc.date.available2022-12-07T15:27:42Z
dc.date.issued2020
dc.description.abstractThe epicardium is the outer mesothelial layer of the heart. It covers the myocardium and plays important roles in both heart development and regeneration. It is derived from the proepicardium (PE), groups of cells that emerges at early developmental stages from the dorsal pericardial layer (DP) close to the atrio-ventricular canal and the venous pole of the heart- tube. In zebrafish, PE cells extrude apically into the pericardial cavity as a consequence of DP tissue constriction, a process that is dependent on Bmp pathway signaling. Expression of the transcription factor Wilms tumor-1, Wt1, which is a leader of important morphogenetic events such as apoptosis regulation or epithelial-mesenchymal cell transition, is also necessary during PE formation. In this study, we used the zebrafish model to compare intensity level of the wt1a reporter line epi:GFP in PE and its original tissue, the DP. We found that GFP is present at higher intensity level in the PE tissue, and differentially wt1 expression at pericardial tissues could be involved in the PE formation process. Our results reveal that bmp2b overexpression leads to enhanced GFP level both in DP and in PE tissueses
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dc.format.extent12es
dc.identifier.citationHistology and Histopathology Vol. 35, nº9 (2020)
dc.identifier.doihttps://doi.org/10.14670/HH-18-238
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/126243
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectZebrafishes
dc.subjectPericardial tissueses
dc.subjectCavalieri's volume estimationes
dc.subjectBmp signalinges
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.1 - Patología del sistema circulatorio, de los vasos sanguíneos. Transtornos cardiovasculareses
dc.titleAnalysis of wt1a reporter line expression levels during proepicardium formation in the zebrafishes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.35, nº9 (2020)