Publication: Analysis of wt1a reporter line expression levels during proepicardium formation in the zebrafish
Authors
Andrés Delgado, Laura ; Galardi Castilla, María ; Mercader, Nadia ; Santamaría, Luis
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-238
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info:eu-repo/semantics/article
Description
Abstract
The epicardium is the outer mesothelial layer
of the heart. It covers the myocardium and plays
important roles in both heart development and
regeneration. It is derived from the proepicardium (PE),
groups of cells that emerges at early developmental
stages from the dorsal pericardial layer (DP) close to the
atrio-ventricular canal and the venous pole of the heart-
tube. In zebrafish, PE cells extrude apically into the
pericardial cavity as a consequence of DP tissue
constriction, a process that is dependent on Bmp
pathway signaling. Expression of the transcription factor
Wilms tumor-1, Wt1, which is a leader of important
morphogenetic events such as apoptosis regulation or
epithelial-mesenchymal cell transition, is also necessary
during PE formation. In this study, we used the zebrafish
model to compare intensity level of the wt1a reporter
line epi:GFP in PE and its original tissue, the DP. We
found that GFP is present at higher intensity level in the
PE tissue, and differentially wt1 expression at pericardial
tissues could be involved in the PE formation process.
Our results reveal that bmp2b overexpression leads to
enhanced GFP level both in DP and in PE tissues
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Citation
Histology and Histopathology Vol. 35, nº9 (2020)
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