Publication: Intestinal uptake of amyloid B protein through columnar epithelial cells in suckling mice
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Date
2009
Authors
Ano, Yasuhisa ; Nakayama, Hiroyuki ; Sakudo, Akikazu ; Sawano, Yoriko ; Tanokura, Masaru ; Itohara, Shigeyoshi ; Onodera, Takashi
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The mechanism of transmission of amyloid
protein, especially the dynamics in the intestine, is still
largely unknown. In the present study, a fusion protein
(Aß-EGFP) that combined enhanced green fluorescent
protein with amyloid-ß protein (Aß) was orally
administered to mice before and after weaning, and the
uptake and kinetics of amyloid protein within the
intestine were elucidated through histopathology. Aß-
EGFP was incorporated into the cytoplasm of columnar
epithelial cells, rather than M cells, at 3 h after
administration and thereafter. Aß-EGFP then
accumulated in the crypt, Peyer's patch, and even the
spleen. However, this uptake was not observed in
weaned mice. These results suggest that a specific
tolerant mechanism for incorporation of Aß escaped
from the digestion exists during suckling periods. This
age-dependent uptake is important for estimating the risk
of transmission.
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