Publication: Glypican-3 (GPC3) expression in human placenta: localization to the differentiated syncytiotrophoblast
Loading...
Date
2001
Authors
Khan, S. ; Blackburn, M. ; Mao, D.L. ; Huber, R. ; Schlessinger, D. ; Fant, M.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The expression of glypican-3 (GPC3), a
heparan-sulfate proteoglycan associated with the
Simpson-Golabi-Behmel fetal overgrowth syndrome,
was studied in normal human placental tissue and cell
lines derived from human placentae. Cytotrophoblasts
derived from term placentae expressed GPC3 mRNA at
low levels in culture. GPC3 mRNA expression increased
markedly during trophoblast differentiation. By contrast,
fibroblast cell lines derived from normal placentae did
not express GPC3 in culture. Similarly, choriocarcinoma
cell lines derived from human placentae (BeWo, JAR,
and JEG) failed to express GPC3 mRNA. In situ
hybridization confirmed the localization of GPC3
mRNA to the syncytiotrophoblast. Furthermore,
immunohistochemical staining of paraffin imbedded
placental tissue demonstrated intense staining of the
syncytiotrophoblast cell layer and less intense staining of
cytotrophoblasts. No staining of mesenchymal elements
was noted. These data confirm the presence of GPC3 in
human placenta and suggest it is expressed by the
differentiated syncytiotrophoblast at term
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.