Publication: Radix Actinidiae chinensis inhibits neovascularization in colorectal cancer and its mechanism
Authors
Minyuan Chen ; Jiante Li ; Jieyu Liu ; Ziqi Meng
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Publisher
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Universidad de Murcia, Departamento de BiologÃa Celular e HistologÃa
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DOI
https://doi.org/10.14670/HH-18-977
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info:eu-repo/semantics/article
Description
Abstract
Objective. Colorectal cancer is one of the
most common cancers worldwide, and its angiogenesis
is a key factor in tumor growth and metastasis. Radix
Actinidiae chinensis is considered to have antitumor
activity in traditional Chinese medicine, but its effect on
neovascularization in colorectal cancer has not been
clarified. Herein, we aimed to evaluate the effect of
different concentrations of Radix Actinidiae chinensis on
the neovascularization of colorectal cancer and explore
its possible mechanisms.
Method. A mouse model of colorectal cancer was
established, and mice were randomly divided into
control, low-, and high-concentration groups. Then the
mice in the experimental group were treated with Radix
Actinidiae chinensis, and its effects on neovascularization
and tumor growth were evaluated by tumor growth curve
tracking, immunohistochemical analysis, vessel density
assessment, RT-qPCR, and protein immunoblotting to
explore the underlying mechanisms.
Results. It was shown that tumor tissues in the high
concentration group exhibited significantly slower
growth in both mass and volume compared with the low
concentration and control groups. Immunohistochemical
staining revealed a reduction in the expression of the
vascular endothelial marker CD31 in the Radix
Actinidiae chinensis treatment group. Moreover, the
protein expression levels of vascular markers in tumor
tissues showed a slight decrease in the low-concentration
group and a marked reduction in the high-concentration
group. These findings suggest that angiogenesis in
the tumor microenvironment was inhibited in a
concentration-dependent manner, with protein
expression levels closely mirroring gene expression
patterns.
Conclusion. The study found that Radix Actinidiae
chinensis inhibits neovascularization in a dose
dependent manner in a mouse model of colorectal
cancer. These results provide experimental support for
its potential use as a therapeutic agent against colorectal
cancer, suggesting that it may suppress tumor growth
and metastasis by inhibiting angiogenesis.
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