Publication: Immunohistochemical changes and atrophy after
chronic ethanol intoxication in rat salivary glands
Authors
Fernandes, Luanna Melo Pereira ; Teixeira, Francisco Bruno ; Junior, Sergio Melo Alves ; Pinheiro, João de Jesus Viana ; Maia, Cristiane Socorro Ferraz ; Lima, Rafael Rodrigues
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-11-604
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info:eu-repo/semantics/article
Description
Abstract
Alcoholism in humans is a chronic and
progressive disease, characterized by loss of ethanol
consumption control. Previous studies have reported that
prolonged exposure to ethanol was responsible for
alterations in glandular tissues of human and rodents.
However, the interrelationship between ethanol and the
glandular system is still the subject of numerous
investigations, including the possible resistance of the
submandibular gland (SG). In the present study, we
investigated whether chronic ethanol exposure during
adolescence may affect the parotid gland (PG) and SG in
female rats. Female rats (n=16) were treated with
distilled water or ethanol (dose of 6.5 g/kg/day, 22.5%
w/v) through gavage for 55 days. Glands were collected,
weighed and submitted to histological processing.
Morphometric analysis was assessed by parenchymal
and stromal area measurements. Smooth muscle actin
(α-SMA), cytokeratin-19 (CK19) and apoptotic caspase3 (CAS) were measured using ImageJ® software.
Chronic ethanol administration did not alter the body
weight of rats after treatment, although it increased
glandular weight (p<0.001), reduced the parenchyma
area (p<0.001) and decreased CK19 and α-SMA
immunostainning in the PG. Besides, ethanol induced
CK19 and CAS overexpression, and the occurrence of
duct-like structures in SG. These results suggest that ethanol induces histological and morphometric changes
in salivary glands of female rats intoxicated with ethanol
during adolescence. Furthermore, the mechanism
underlying these alterations needs to be investigated but
may be not related to the inflammatory process.
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Citation
Histology and histopathology, Vol. 30, nº 9 (2015)
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Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/