Publication:
Targeting AURKA by microRNA-490-3p suppresses gastric cancer cell growth

Thumbnail Image
Files
Chen-37-397-404-2022.pdf(4.3 MB)
Date
2022
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Chen, Rui ; Zhou, Shenkang ; Fang, Chengfeng ; Ye, Feifei ; Chen, Jianhui ; Jiang, Pinlu
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
publication.page.editor
publication.page.department
DOI
https://doi.org/ 10.14670/HH-18-415
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Objective. To illustrate the molecular mechanism of microRNA-490-3p regulating gastric cancer (GC) cells by targeting AURKA. Methods. Genes with significantly different expression in GC and normal tissue in TCGA-STAD dataset were analyzed by bioinformatics. Expression levels of genes and proteins in GC cells were measured by qRT-PCR and western blot. The interaction between microRNA-490-3p and AURKA was verified by dual luciferase assay. Proliferation, migration, invasion and apoptosis of GC cells were evaluated through a set of cell function assays. Results. MicroRNA-490-3p was significantly less expressed in GC, while AURKA was significantly highly expressed. Dual luciferase reporter gene assay proved that microRNA-490-3p targeted AURKA. Upregulation of microRNA-490-3p restrained proliferation, migration, invasion and stimulated apoptosis of GC cells, which was attenuated by overexpression of AURKA. Conclusions. MicroRNA-490-3p was likely to restrain the development of GC cells by inhibiting AURKA, and it may be an underlying target for GC treatment
publication.page.subject
microRNA-490-3p , AURKA , Proliferation , Invasion , Gastric cancer , Migration
Citation
Histology and Histopathology Vol. 37, nº4 (2022)
URI
http://hdl.handle.net/10201/128454
item.page.embargo
Collections
Histology and histopathology Vol.37, nº4 (2022)
Ir a Estadísticas