DigitalUM :: Browsing by Subject "AURKA"

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Open Access
Targeting AURKA by microRNA-490-3p suppresses gastric cancer cell growth
(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2022) Chen, Rui; Zhou, Shenkang; Fang, Chengfeng; Ye, Feifei; Chen, Jianhui; Jiang, Pinlu
Objective. To illustrate the molecular mechanism of microRNA-490-3p regulating gastric cancer (GC) cells by targeting AURKA. Methods. Genes with significantly different expression in GC and normal tissue in TCGA-STAD dataset were analyzed by bioinformatics. Expression levels of genes and proteins in GC cells were measured by qRT-PCR and western blot. The interaction between microRNA-490-3p and AURKA was verified by dual luciferase assay. Proliferation, migration, invasion and apoptosis of GC cells were evaluated through a set of cell function assays. Results. MicroRNA-490-3p was significantly less expressed in GC, while AURKA was significantly highly expressed. Dual luciferase reporter gene assay proved that microRNA-490-3p targeted AURKA. Upregulation of microRNA-490-3p restrained proliferation, migration, invasion and stimulated apoptosis of GC cells, which was attenuated by overexpression of AURKA. Conclusions. MicroRNA-490-3p was likely to restrain the development of GC cells by inhibiting AURKA, and it may be an underlying target for GC treatment