Publication: Immunocytohistochemical characterization of pituitary cells of the bluefin tuna, Thunnus thynnus L
Authors
Rodriguez-Gomez, F.J. ; Rendón-Unceta, M.C. ; Piñuela, C. ; Muñoz-Cueto, J.A. ; Sarasquete, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In this paper we report the first complete
mapping of the pituitary in a tuna species. The various
different adenohypophysis cell types of the bluefin tuna,
Thunnus thynnus L. have been identified and located
using different antisera against mammalian and piscine
hormones and various histochemical techniques: PAS,
Alcian Blue pH 2.5 and lectins -ConA and WGA-
(Neutral and Acidic Glycoproteins); Bromophenol Blue
(Proteins) and Tioglycollate-Ferric-Ferricianide-Fe111 (-
S-S- groups). Prolactin (PRL) and adrenocorticotrophic
(ACTH) cells were located in the rostral pars distalis
(RPD) of the pituitary, while the proximal pars distalis
(PPD) displayed gonadotrophic (GTH), thyrotrophic
(TSH), somatotrophic (GH) and also a few PRL cells.
Moreover, somatolactin (SL) and melanotrophic (MSH)
cells were identified inside the pars intermedia (PI).
Interestingly, some SL-immunoreactive fibers were dso
detected in the neurohypophysis. Some GTH cells were
also located on the exterior surface of the PI.
Glycoproteins containing mannose (Man) andlor glucose
(Glc); N-acetyl-glucosamine (GlcNAc) andlor sialic acid
sugar residues, as well as -S-S- groups, were observed in
GTH, TSH and SL cells. The Bromophenol Blue
technique stained amphiphilic SL, acidophilic GH cells
and weakly ACTH cells. GH and ACTH cells were
unreactive to PAS, Alcian Blue, Tioglycollate-Ferric-
Ferricianide-Fe111 and lectin (Con A and WGA)
techniques. Finally, PAS reaction was positive in
amphiphilic SL cells, which were PbH unreactive, while
MSH and ACTH cells were stained with PbH technique
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