Browsing by Subject "Tuna"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    Open Access
    Immunocytohistochemical characterization of pituitary cells of the bluefin tuna, Thunnus thynnus L
    (Murcia : F. Hernández, 2001) Rodriguez-Gomez, F.J.; Rendón-Unceta, M.C.; Piñuela, C.; Muñoz-Cueto, J.A.; Sarasquete, C.
    In this paper we report the first complete mapping of the pituitary in a tuna species. The various different adenohypophysis cell types of the bluefin tuna, Thunnus thynnus L. have been identified and located using different antisera against mammalian and piscine hormones and various histochemical techniques: PAS, Alcian Blue pH 2.5 and lectins -ConA and WGA- (Neutral and Acidic Glycoproteins); Bromophenol Blue (Proteins) and Tioglycollate-Ferric-Ferricianide-Fe111 (- S-S- groups). Prolactin (PRL) and adrenocorticotrophic (ACTH) cells were located in the rostral pars distalis (RPD) of the pituitary, while the proximal pars distalis (PPD) displayed gonadotrophic (GTH), thyrotrophic (TSH), somatotrophic (GH) and also a few PRL cells. Moreover, somatolactin (SL) and melanotrophic (MSH) cells were identified inside the pars intermedia (PI). Interestingly, some SL-immunoreactive fibers were dso detected in the neurohypophysis. Some GTH cells were also located on the exterior surface of the PI. Glycoproteins containing mannose (Man) andlor glucose (Glc); N-acetyl-glucosamine (GlcNAc) andlor sialic acid sugar residues, as well as -S-S- groups, were observed in GTH, TSH and SL cells. The Bromophenol Blue technique stained amphiphilic SL, acidophilic GH cells and weakly ACTH cells. GH and ACTH cells were unreactive to PAS, Alcian Blue, Tioglycollate-Ferric- Ferricianide-Fe111 and lectin (Con A and WGA) techniques. Finally, PAS reaction was positive in amphiphilic SL cells, which were PbH unreactive, while MSH and ACTH cells were stained with PbH technique
  • Thumbnail Image
    Publication
    Open Access
    Valoración del riesgo vírico asociado a la alimentación con carnada en el engorde del atún rojo (Thunnus Thynnus)
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2012) Peñalver García, José; María Dolores Pedrero, Emilio; Tafalla, C.; Bermúdez, L.; Gómez, O.; Viuda, E.; Romero, E.; Ciencias Sociosanitarias
    El engorde de atún rojo es una actividad económica de gran importancia en el contexto de la acuicultura nacional, especialmente para el área mediterránea. La alimentación se realiza exclusivamente mediante carnada compuesta por ejemplares completos de peces de pequeños pelágicos y moluscos cefalópodos como el calamar. La cantidad de carnada necesaria para alimentar a estos animales es muy considerable, ya que se estima entre el 2 y el 10% del peso corporal de los atunes. La D. G. de Ganadería y Pesca, dentro programa Piloto de Vigilancia Epidemiológica en Animales Acuáticos en aguas interiores de la Región de Murcia iniciado en el 2006, establece la realización de una serie de controles regulares sobre la carnada utilizada en la granjas de atunes del Litoral de Murcia. El objetivo es valorar de forma práctica la posibilidad de transmisión de enfermedades víricas a la fauna silvestre local y al resto de granjas de acuicultura situadas en la zona. En el presente trabajo se reflejan los resultados de los 4 años de este programa de vigilancia.