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The importance of vascular epithelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) in rhinitis medicamentosa pathogenesis: An experimental rat model study

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dc.contributor.authorCumbul, Alev
dc.contributor.authorBulut, Fuat
dc.date.accessioned2023-02-14T09:55:22Z
dc.date.available2023-02-14T09:55:22Z
dc.date.issued2022
dc.description.abstractObjective. Our aim in this study is to reveal the expression of Vascular Endothelial Growth Factor (VEGF) and inducible Nitric Oxide Synthase (iNOS) in the pathogenesis of rhinitis medicamentosa (RM), which occurs as a result of the overdose and long-term use of topical nasal decongestants. Methods. In this study, 24 Wistar albino rats were divided into two groups as experimental and control groups. In the experimental group, 50 µl of 0.05% oxymetazoline (iliadin® merck) was applied intranasally to each nostril three times a day for 2 months with the help of a micropipette. 50 µl saline was applied to the control group. At the end of the second month, the rats were examined. RM was detected in the experimental group. Then the nasal tissues of the rats were removed and fixed with 10% phosphate buffered neutral formaldehyde (pH=7.4). Nasal tissues were decalcified in Morse's solution (10% sodium citrate and 22.5% formic acid). Histopathological evaluations of the preparations were stained using Masson Trichrome (TCM) and Hematoxylin Eosin (H&E) techniques and immunohistochemical examinations of the preparations were stained with VEGF and iNOS antibodies and photographed using the Leica DM6000B microscope and the Leica Application Suite Program. Results. In the RM group, we found a significant increase in VEGF and iNOS expression in the nasal mucosa compared to the control group (p<0.001). We also observed the main histopathological changes in the nasal mucosa under a light microscope, including squamous metaplasia in the epithelium of the tunica mucosa, submucosal perivascular edema and degeneration of the submucosal glands. Conclusions. According to these results, increased expression levels of VEGF and iNOS play an important role in rebound swelling in RM pathogenesis.es
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dc.identifier.citationHistology and Histopathology Vol. 37, nº3 (2022)
dc.identifier.doihttps://doi.org/10.14670/HH-18-399
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/128404
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectRhinitises
dc.subjectOxymetazolinees
dc.subjectNasal mucosaes
dc.subjectEdemaes
dc.subjectVascular Endothelial Growth Factor Aes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleThe importance of vascular epithelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) in rhinitis medicamentosa pathogenesis: An experimental rat model studyes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.37, nº3 (2022)