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MiR-195-5p suppresses gastric adenocarcinoma cell progression via targeting OTX1

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dc.contributor.authorHu, Sizhe
dc.contributor.authorZhou, Huanting
dc.contributor.authorZhao, Xiaokang
dc.contributor.authorQian, Feng
dc.contributor.authorJin, Cancan
dc.date.accessioned2023-05-24T15:55:58Z
dc.date.available2023-05-24T15:55:58Z
dc.date.issued2023
dc.description.abstractGastric adenocarcinoma (GAC) caused by malignant transformation of gastric adenocytes is a malignancy with high incidence. MiR-195-5p modulates a variety of cancers. One of its target genes, orthodenticle homeobox 1 (OTX1), is believed to be a key modulator of tumor progression. We aim to analyze the mechanism of miR-195-5p and OTX1 in GAC. MiR195-5p and OTX1 mRNA levels in GAC cells were tested via qRT-PCR. OTX1 protein and EMT-related protein levels were examined through western blot. Several cell functional assays were designed to measure changes in cell malignant behaviors. Dual luciferase assay verified the targeting relation of miR-195-5p and OTX1. These experimental results showed significantly low miR-195-5p expression and significantly high OTX1 expression in GAC cells. Enforced miR-195-5p level repressed cell malignant progression and accelerated cell apoptosis in GAC. Increased OTX1 weakened the above-mentioned effect caused by overexpressing miR-195-5p. Thus, miR-195-5p restrained migration, proliferation, invasion and epithelial-mesenchymal transition process of GAC cells, and promoted cell apoptosis through regulating OTX1. A new insight is provided for searching for biomarkers or therapeutic targets of GAC.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology Vol. 38, nº6 (2023)
dc.identifier.doihttps://doi.org/10.14670/HH-18-512
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/131392
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectmiR-195-5pes
dc.subjectGastric adenocarcinomaes
dc.subjectOTX1es
dc.subjectInvasiones
dc.subjectMigrationes
dc.subjectProliferationes
dc.subjectEpithelialmesenchymal transitiones
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleMiR-195-5p suppresses gastric adenocarcinoma cell progression via targeting OTX1es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.38, nº6 (2023)