Publication:
MiR-195-5p suppresses gastric adenocarcinoma cell progression via targeting OTX1

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Hu-38-659-668-2023.pdf(6.84 MB)
Date
2023
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Authors
Hu, Sizhe ; Zhou, Huanting ; Zhao, Xiaokang ; Qian, Feng ; Jin, Cancan
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-512
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info:eu-repo/semantics/article
Description
Abstract
Gastric adenocarcinoma (GAC) caused by malignant transformation of gastric adenocytes is a malignancy with high incidence. MiR-195-5p modulates a variety of cancers. One of its target genes, orthodenticle homeobox 1 (OTX1), is believed to be a key modulator of tumor progression. We aim to analyze the mechanism of miR-195-5p and OTX1 in GAC. MiR195-5p and OTX1 mRNA levels in GAC cells were tested via qRT-PCR. OTX1 protein and EMT-related protein levels were examined through western blot. Several cell functional assays were designed to measure changes in cell malignant behaviors. Dual luciferase assay verified the targeting relation of miR-195-5p and OTX1. These experimental results showed significantly low miR-195-5p expression and significantly high OTX1 expression in GAC cells. Enforced miR-195-5p level repressed cell malignant progression and accelerated cell apoptosis in GAC. Increased OTX1 weakened the above-mentioned effect caused by overexpressing miR-195-5p. Thus, miR-195-5p restrained migration, proliferation, invasion and epithelial-mesenchymal transition process of GAC cells, and promoted cell apoptosis through regulating OTX1. A new insight is provided for searching for biomarkers or therapeutic targets of GAC.
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miR-195-5p , Gastric adenocarcinoma , OTX1 , Invasion , Migration , Proliferation , Epithelialmesenchymal transition
Citation
Histology and Histopathology Vol. 38, nº6 (2023)
URI
http://hdl.handle.net/10201/131392
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Histology and histopathology Vol.38, nº6 (2023)
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