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Microarray analysis of Myf5-/-:MyoD-/- hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiation

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dc.contributor.authorBaguma-Nibasheka, M.es
dc.contributor.authorAngka, H.E.es
dc.contributor.authorInanlou, M.R.
dc.contributor.authorKablar, B.
dc.date.accessioned2012-05-21T12:09:29Z
dc.date.available2012-05-21T12:09:29Z
dc.date.issued2007
dc.description.abstractFetal breathing-like movements (FBMs) are important in normal lung growth and pneumocyte differentiation. In amyogenic mouse embryos (designated as Myf5-/-:MyoD-/-, entirely lacking skeletal musculature and FBMs), type II pneumocytes fail to differentiate into type I pneumocytes, the cells responsible for gas exchange, and the fetuses die from asphyxia at birth. Using oligonucleotide microarrays, we compared gene expression in the lungs of Myf5-/- :MyoD-/- embryos to that in normal lungs at term. Nine genes were found to be up-regulated and 54 downregulated at least 2-fold in the lungs of double-mutant embryos. Since many down-regulated genes are involved in lymphocyte function, immunohistochemistry was employed to study T- and B-cell maturity in the thymus and spleen. Our findings of normal lymphocyte maturity implied that the down-regulation was specific to the double-mutant lung phenotype and not to its immune system. Immunostaining also revealed altered distribution of transcription and growth factors (SATB1, c-Myb, CTGF) from down-regulated genes whose knockouts are now known to undergo embryonic or neonatal death secondary to respiratory failure. Together, it appears that microarray analysis has identified a profile of genes potentially involved in pneumocyte differentiation and therefore in the mechanisms that may be implicated in the mechanochemical signal transduction pathways underlying FBMs-dependent pulmonary hypoplasia.es
dc.formatapplication/pdfes
dc.format.extent13es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/27580
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectPneumocyteses
dc.subjectPulmonary hypoplasiaes
dc.subject.other61 - Medicinaes
dc.titleMicroarray analysis of Myf5-/-:MyoD-/- hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiationes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.22, nº 5 (2007)