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Histopathological alterations, EROD activity, CYP1A protein and biliary metabolites in gilthead seabream Sparus aurata exposed to Benzo(a)pyrene

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dc.contributor.authorOrtiz-Delgado, J.B.es
dc.contributor.authorSegner, H.es
dc.contributor.authorArellano, J.M.
dc.contributor.authorSarasquete, C.
dc.date.accessioned2012-05-21T12:09:12Z
dc.date.available2012-05-21T12:09:12Z
dc.date.issued2007
dc.description.abstractThis study compared for seabream, Sparus aurata exposed to benzo(a)pyrene-B(a)P-, the response of molecular cytochrome P450 1A (CYP1A) and cellular histopathology biomarkers. Male gilthead seabream, Sparus aurata specimens were exposed for 20 days via water to a series of high B(a)P concentrations. CYP1A was assessed by measuring enzymatic activity (EROD) and CYP1A protein content, and cellular responses were evaluated by routine histopathological methods. In addition, biliary metabolites were measured in order to verify that B(a)P was absorbed and metabolised. Histological lesions, both in liver and gills, increased in parallel to B(a)P concentrations, with the majority of changes representing rather non-specific alterations. Hepatic EROD and CYP1A proteins data showed a concentration-dependent induction, while in the gills, EROD activity but not CYP1A proteins showed a nonmonotonous dose response, with a maximum induction level at 200 μ g B(a)P.L-1 and decreasing levels thereafter. The findings provide evidence that short-term, high dose exposure of fish can result in significant uptake and metabolism of the lipophilic B(a)P, and in pronounced pathological damage of absorptive epithelia and internal organs.es
dc.formatapplication/pdfes
dc.format.extent16es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/27567
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectHistopathologyes
dc.subjectSparus aurataes
dc.subject.other615 - Farmacología. Terapéutica. Toxicología. Radiologíaes
dc.titleHistopathological alterations, EROD activity, CYP1A protein and biliary metabolites in gilthead seabream Sparus aurata exposed to Benzo(a)pyrenees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.22, nº 4 (2007)