Publication: Histopathological alterations, EROD activity, CYP1A protein and biliary metabolites in gilthead seabream Sparus aurata exposed to Benzo(a)pyrene
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Date
2007
Authors
Ortiz-Delgado, J.B. ; Segner, H. ; Arellano, J.M. ; Sarasquete, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This study compared for seabream, Sparus
aurata exposed to benzo(a)pyrene-B(a)P-, the response
of molecular cytochrome P450 1A (CYP1A) and cellular
histopathology biomarkers. Male gilthead seabream,
Sparus aurata specimens were exposed for 20 days via
water to a series of high B(a)P concentrations. CYP1A
was assessed by measuring enzymatic activity (EROD)
and CYP1A protein content, and cellular responses were
evaluated by routine histopathological methods. In
addition, biliary metabolites were measured in order to
verify that B(a)P was absorbed and metabolised.
Histological lesions, both in liver and gills, increased in
parallel to B(a)P concentrations, with the majority of
changes representing rather non-specific alterations.
Hepatic EROD and CYP1A proteins data showed a
concentration-dependent induction, while in the gills,
EROD activity but not CYP1A proteins showed a nonmonotonous
dose response, with a maximum induction
level at 200 μ g B(a)P.L-1 and decreasing levels
thereafter. The findings provide evidence that short-term,
high dose exposure of fish can result in significant
uptake and metabolism of the lipophilic B(a)P, and in
pronounced pathological damage of absorptive epithelia
and internal organs.
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