Publication: Expression profiles of angiogenesis in two high grade chondrosarcomas: A xenotransplant experience in nude mice
Authors
Giner, Francisco ; López Guerrero, José Antonio ; Machado, Isidro ; García Casado, Zaida ; Fernández Serra, Antonio ; Peydró Olaya, Amando ; Llombart Bosch, Antonio
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-880
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info:eu-repo/semantics/article
Description
Abstract
Background. Chondrosarcomas (Chs) are
malignant cartilage-forming tumors that represent the
third most common malignant solid tumor of bone in
adults. Angiogenesis is a major factor for tumor growth
and metastasis. Our aim was to make a histological,
immunohistochemical, ultrastructural and molecular
characterization of the neovascularization established
between xenotransplanted Chs and the host during the
initial phases of growth in nude transfer, in order to find
potential markers for distinguishing between high grades
II and III Chs. Methods. two xenotransplanted high
grade human Chs were evaluated. Tumor pieces were
implanted subcutaneously on the backs of 14 athymic
Balb-c nude mice. The animals were sacrificed 24, 48,
and 96 hours; and 7, 14, 21 and 28 days after implantation. Two grade I Chs were also transferred in nude but
did not grow. Results. Morphological differences were
apparent between these two Chs during the early stages
of neoplastic growth. Immunohistochemistry demonstrated overexpression of pro-angiogenic factors 24h-48h
after tumor implantation. Additionally, neoplastic cells
co-expressed chemokines (CXCL9, CXCL10 and GRO)
and their receptors. Molecular studies showed two
expression profiles, revealing an early and a late phase in
the angiogenic process. Conclusion. High grade Chs
demonstrated two different stages of induced angiogenesis, with an intimate association between structural
and molecular events that might explain the different
aggressive biological behavior of grade II and III Chs.
The present model may be useful for testing the effect of
anti-angiogenic drugs.
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Citation
Histology and Histopathology, Vol.32, nº12, (2017)
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