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In vitro antigen trapping by gil1 cells of the rainbow trout: an immunohistochemical study

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dc.contributor.authorTorroba, M.es
dc.contributor.authorAnderson, D.P.es
dc.contributor.authorDixon, O.W.
dc.contributor.authorCasares, F.
dc.contributor.authorVaras, A.
dc.contributor.authorAlonso, L.M.
dc.date.accessioned2011-02-01T09:16:33Z
dc.date.available2011-02-01T09:16:33Z
dc.date.issued1993
dc.description.abstractAn in vitro assay was used to study the involvement of gill cells in the trapping and processing of particulate antigens. Gills were routinely processed for light microscopy after being placed in medium containing either Yersinia ruckeri O-antigen-labelled fluorescent beads, unlabelled fluorescent beads, Y. ruckeri O-antigen or formalin-killed Y. ruckeri, for 0, 30 S, 1, 5 and 30 min. Y. ruckeri formalin-killed cells, Y. ruckeri O-antigen and fluorescent beads labelled with Y. ruckeri O-antigen were taken in by gill epithelial cells as soon as 30 S after administration. In contrast, unlabelled fluorescent beads adhered to the epithelial cell membranes, but did not occur inside the gill cells. These results are discussed principally in relationship with the specificity of antigen trapping.es
dc.formatapplication/pdfes
dc.format.extent5es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/18406
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectAntigen trappinges
dc.subjectRainbow troutes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleIn vitro antigen trapping by gil1 cells of the rainbow trout: an immunohistochemical studyes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol. 8, nº 2 (1993)