Publication: In vitro antigen trapping by gil1 cells of the rainbow trout: an immunohistochemical study
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Date
1993
Authors
Torroba, M. ; Anderson, D.P. ; Dixon, O.W. ; Casares, F. ; Varas, A. ; Alonso, L.M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
An in vitro assay was used to study the
involvement of gill cells in the trapping and processing
of particulate antigens. Gills were routinely processed
for light microscopy after being placed in medium
containing either Yersinia ruckeri O-antigen-labelled
fluorescent beads, unlabelled fluorescent beads, Y.
ruckeri O-antigen or formalin-killed Y. ruckeri, for 0,
30 S, 1, 5 and 30 min. Y. ruckeri formalin-killed cells, Y.
ruckeri O-antigen and fluorescent beads labelled with Y.
ruckeri O-antigen were taken in by gill epithelial cells as
soon as 30 S after administration. In contrast, unlabelled
fluorescent beads adhered to the epithelial cell
membranes, but did not occur inside the gill cells. These
results are discussed principally in relationship with the
specificity of antigen trapping.
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