Publication:
Modulatory role of IL10 in endothelial cell damage and platelet adhesion

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Modulatory role of IL10 in endothelial.pdf(3.55 MB)
Date
2003
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Authors
Gimeno, M.J. ; Pascual, G. ; García-Honduvilla, N. ; Prieto, A. ; Alvarez de Mon, M. ; Bellón, J.M. ; Buján, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This study explores the possibility of a regulatory role for cytokine IL-10 in platelet aggregation as an active vascular repair mechanism. Endothelial cells from human umbilical cord vein were cultured in the presence of different IL-10 concentrations (0-100 ng/ml). Platelet-rich plasma was then added to these cultures and allowed to act for 30 minutes. To rule out blood plasma involvement, washed platelets were also incubated with IL-10 (0-100 ng/ml). Changes in endothelial cell morphology were observed depending on the IL-10 concentration used; apoptotic cells appearing at the highest IL-10 concentration. Greatest platelet adhesion was noted at the highest IL-10 concentration. It was concluded that, in this in vitro model, low IL-10 levels do not affect cell viability or the pattern of platelet adhesion, but at high doses, this cytokine induces cell death and enhances platelet deposition.
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Endothelial cells , Platelet
Citation
URI
http://hdl.handle.net/10201/21475
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Collections
Histology and histopathology Vol.18, nº 3 (2003)
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