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Immunohistochemical study of the upper surface layer in rat mandibular condylar cartilage

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dc.contributor.authorZea-Aragón, Z.es
dc.contributor.authorOhtsuki, K.es
dc.contributor.authorOhnishi, M.
dc.contributor.authorOhno, S.
dc.date.accessioned2011-06-08T09:04:54Z
dc.date.available2011-06-08T09:04:54Z
dc.date.issued2004
dc.description.abstractBoth hyaluronic acid and fibronectin localizations were examined in the upper surface layer of rat mandibular condylar cartilages by immunohistochemical techniques. Their delicate structure was successfully preserved by preparation procedures of joint condyles with disks. Paraformaldehyde-fixed cartilaginous tissues were cut in a cryostat, and cryosections were analyzed using streptavidinperoxidase and indirect immunofluorescence methods. Another immunogold method with conventional preparation procedures and a quick-freezing method was performed for their ultrastructural analyses. Both hyaluronic acid-binding protein and anti-fibronectin antibody were used to localize hyaluronic acid and fibronectin in the mandibular condylar cartilage, respectively. Some cryosections were pre-treated with hyaluronidase and chondroitinase before such labeling. The upper surface layer was composed of double laminar structures. One bordered with the cartilage matriceal surface, which was positive for fibronectin. The hyaluronic acid was localized over the fibronectin layer. Therefore, the hyaluronic acid in vivo was bound with fibronectin in the cartilaginous matrix, performing lubrication for the mandibular joint movement.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/21542
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectArticular cartilagees
dc.subjectFibronectines
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::611 - Anatomíaes
dc.titleImmunohistochemical study of the upper surface layer in rat mandibular condylar cartilagees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.19, nº 1 (2004)