Publication: Immunohistochemical study of the upper surface layer in rat mandibular condylar cartilage
Authors
Zea-Aragón, Z. ; Ohtsuki, K. ; Ohnishi, M. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Both hyaluronic acid and fibronectin
localizations were examined in the upper surface layer of
rat mandibular condylar cartilages by immunohistochemical
techniques. Their delicate structure was
successfully preserved by preparation procedures of joint
condyles with disks. Paraformaldehyde-fixed
cartilaginous tissues were cut in a cryostat, and
cryosections were analyzed using streptavidinperoxidase
and indirect immunofluorescence methods.
Another immunogold method with conventional
preparation procedures and a quick-freezing method was
performed for their ultrastructural analyses. Both
hyaluronic acid-binding protein and anti-fibronectin
antibody were used to localize hyaluronic acid and
fibronectin in the mandibular condylar cartilage,
respectively. Some cryosections were pre-treated with
hyaluronidase and chondroitinase before such labeling.
The upper surface layer was composed of double
laminar structures. One bordered with the cartilage
matriceal surface, which was positive for fibronectin.
The hyaluronic acid was localized over the fibronectin
layer. Therefore, the hyaluronic acid in vivo was bound
with fibronectin in the cartilaginous matrix, performing
lubrication for the mandibular joint movement.
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