Publication: Expression of glutathione S-transferase T1 GSTT1 in human brain tumours
Loading...
Date
2006
Authors
Diedrich, A. ; Bock, H.C. ; König, F. ; Schulz, T.G. ; Ludwig, H.C. ; Herken, R. ; Quondamatteo, F.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Glutathione S-transferases (GSTs) play a
central role in a number of metabolic processes.
Glutathione S-transferase T1 (GSTT1) is a polymorphic
cytosolic enzyme and a member of the theta class of
GSTs. Typical substrates for GSTT1 are industrial
compounds, such as dichloromethane and ethylene
oxide. It has been shown that also chemotherapeutic
drugs such as BCNU [i.e. 1,3-bis(2-chloroethyl)-1-
nitrosourea] are efficiently inactivated by GSTT1.
BCNU is a drug which is increasingly used locally in the
chemotherapy of glioblastoma multiforme WHO grade
IV. Therefore, if GSTT1 were expressed in neoplastic
cells of brain tumours it could be a factor for
chemoresistance. In order to clarify a possible role of
GSTT1 in chemoresistance, as a first step, we localized
this enzyme in malignant gliomas such as glioblastoma
multiforme WHO grade IV and oligodendroglioma
WHO grade II. Because of its polymorphism we first
genotyped the samples for GSTT1 by PCR. Using in situ
hybridization, we then demonstrated that GSTT1 transcripts are expressed in neoplastic cells of both
tumour types. Immunohistochemistry revealed then that
whereas neoplastic cells in glioblastoma multiforme
WHO grade IV contain GSTT1, it was not localized in
oligodendroglioma cells. Given the polymorphism of
GSTT1 and its potential activity towards BCNU, the
localization of GSTT1 in glioblastoma cells can be
considered as a possible factor of non-homogeneous
chemotherapy response among patients with different
GSTT1 genotypes.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.