Publication: Effect of dolomite on the repair of bone defects in rats; histological study
Loading...
Date
2010
Authors
Moreschi, Eduardo ; Hernandes, Luzmarina ; Araujo Dantas, Jailson ; Raffaini Covas Pereira da Silva, Maria Angélica ; Casaroto, Ana Regina ; Bersani-Amado, Ciomar Aparecida
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The aim of the present study was to evaluate
histologically and radiographically the tissue response to
dolomite [CaMg(CO3)2] and its osteogenic potential in
the repair of bone cavities in the calvaria of rats. A bone
defect 10 mm in diameter and 1 mm deep was made in
the calvaria of male Wistar rats. The defects were filled
with dolomite, inorganic bovine bone (positive control),
or coagulum (negative control). The animals were
euthanized 7, 15, 30, and 60 days after surgery, and
specimens were collected for radiographic and
microscopic analyses. The bone defects were processed
for paraffin embedding and H&E staining. The
histological study revealed that dolomite stimulated a
moderate inflammatory response, with programmed cell
death in the first 15 days, compared to bovine bone
which showed a moderate to intense acute response. In
the chronic phase, the inflammatory response was
characterized by the occurrence of macrophages
organized as epithelioid cells in the dolomite group, and
giant cells in the bovine-bone group. Fibrosis developed
in all three groups; however, encapsulation of the
fragments, reabsorption, and osteoconductive activity
occurred only in the defects filled with bovine bone. The
radiographic analysis showed that the bovine bone was
most efficient in the repair of the defects, followed by
the dolomite and the coagulum. This study demonstrated
that the dolomite stimulated a moderate acute
inflammatory response with programmed cell death, and
a chronic inflammatory response by means of the
phagocytic mononuclear system. Although osteoconductive
activity was not shown, the dolomite favored
the repair process, compared to the coagulum group.
publication.page.subject
Citation
item.page.embargo
Ir a EstadĂsticas
Sin licencia Creative Commons.