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Markers of squamocolumnar junction cells in normal tonsils and oropharyngeal cancer with and without HPV infection

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dc.contributor.authorMorbini, Patrizia
dc.contributor.authorCapello, Gianluca
dc.contributor.authorAlberizzi, Paola
dc.contributor.authorBenazzo, Marco
dc.contributor.authorPaglino, Chiara
dc.contributor.authorComoli, Patrizia
dc.contributor.authorPedrazzoli, Paolo
dc.date.accessioned2020-09-29T11:27:27Z
dc.date.available2020-09-29T11:27:27Z
dc.date.issued2015
dc.description.abstracty. HPV infection has been identified recently as the causative agent of a subset of squamous cell carcinomas arising in oropharyngeal tonsils. Factors influencing the susceptibility of tonsillar epithelium to HPV-induced oncogenesis are far from being elucidated. A 5-protein signature including cytokeratin (CK)7, anterior gradient (AGR)2, cluster differentiation (CD)63, matrix metalloproteinase (MMP)7, and guanine deaminase (GDA) has recently been found to identify a residual embryonic cell population in the squamocolumnar (SC) junction of the cervix, susceptible to HPV infection, and cancers originating from these cells. The expression of SC junction markers was investigated with immunohistochemistry in normal tonsils and in oropharyngeal carcinomas (OPC) fully characterised for HPV. All markers were constantly expressed in the reticulated epithelial cells of the tonsillar crypts, with variable diffusion and intensity; in OPC, positivity was observed in 36.5%, 29.2%, 39%, 17%, and 25% of cases with respectively AGR2, CK7, GDA, CD63, and MMP7 antibodies. No OPC was positive for all markers; 6 were completely negative. AGR2 and CK7 showed significant association with tumor- and HPV-related parameters. AGR2 expression was associated with tumor origin in the tongue base (p=0.013); CK7 was associated withnon-keratinising morphology (p=0.013). p16 tumor cell expression was associated with AGR2 (p=0.021); transcriptionally active HPV infection was associated with AGR2 and CK7 (p=0.024 and 0.043). Expression of SC junction markers in tonsillar crypt cells might be related to the embryological development of tonsillar structures; their partial association with HPV oncogenic infection could help to identify HPVsusceptible cells and related OPC.es
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dc.format.extent7es
dc.identifier.citationHistology and Histopathology, vol. 30, nÂş 7, (2015)
dc.identifier.doi10.14670/HH-11-590
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/96678
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de BiologĂ­a Celular e HistologĂ­aes
dc.relationSin financiaciĂłn externa a la universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHPV-associated oropharyngeal carcinomaes
dc.subjectSquamocolumnar junctiones
dc.subjectTonsillar cryptes
dc.subjectAnterior gradient 2es
dc.subjectCytokeratin 7es
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - PatologĂ­a. Medicina clĂ­nica. OncologĂ­aes
dc.titleMarkers of squamocolumnar junction cells in normal tonsils and oropharyngeal cancer with and without HPV infectiones
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30, nÂş7 (2015)