Publication: Markers of squamocolumnar junction cells in normal tonsils and oropharyngeal cancer with and without HPV infection
Authors
Morbini, Patrizia ; Capello, Gianluca ; Alberizzi, Paola ; Benazzo, Marco ; Paglino, Chiara ; Comoli, Patrizia ; Pedrazzoli, Paolo
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
publication.page.editor
publication.page.department
DOI
10.14670/HH-11-590
item.page.type
info:eu-repo/semantics/article
Description
Abstract
y. HPV infection has been identified recently as
the causative agent of a subset of squamous cell
carcinomas arising in oropharyngeal tonsils. Factors
influencing the susceptibility of tonsillar epithelium to
HPV-induced oncogenesis are far from being elucidated.
A 5-protein signature including cytokeratin (CK)7,
anterior gradient (AGR)2, cluster differentiation (CD)63,
matrix metalloproteinase (MMP)7, and guanine
deaminase (GDA) has recently been found to identify a
residual embryonic cell population in the squamocolumnar (SC) junction of the cervix, susceptible to
HPV infection, and cancers originating from these cells.
The expression of SC junction markers was investigated
with immunohistochemistry in normal tonsils and in
oropharyngeal carcinomas (OPC) fully characterised for
HPV.
All markers were constantly expressed in the
reticulated epithelial cells of the tonsillar crypts, with
variable diffusion and intensity; in OPC, positivity was
observed in 36.5%, 29.2%, 39%, 17%, and 25% of cases
with respectively AGR2, CK7, GDA, CD63, and MMP7
antibodies. No OPC was positive for all markers; 6 were
completely negative. AGR2 and CK7 showed significant
association with tumor- and HPV-related parameters.
AGR2 expression was associated with tumor origin in
the tongue base (p=0.013); CK7 was associated withnon-keratinising morphology (p=0.013). p16 tumor cell
expression was associated with AGR2 (p=0.021);
transcriptionally active HPV infection was associated
with AGR2 and CK7 (p=0.024 and 0.043).
Expression of SC junction markers in tonsillar crypt
cells might be related to the embryological development
of tonsillar structures; their partial association with HPV
oncogenic infection could help to identify HPVsusceptible cells and related OPC.
publication.page.subject
Citation
Histology and Histopathology, vol. 30, nÂş 7, (2015)
item.page.embargo
Ir a EstadĂsticas
Este Ătem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/