Publication: A comparative study on the effects of bfluorouracll on glycosaminoglycan synthesis during palate development in quail and hamster
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Date
1994
Authors
Young, A.V. ; Hehn, B.M. ; Cheng, K. M. ; Shah, R.M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
A comparative study was undertaken to
investigate the effects of 5-fluorouracil (FU) on
glycosaminoglycans (GAG) synthesis during morphogenesis
of the secondary palate in birds (where, unlike
mammals, palate morphogenesis begins in a horizontal
direction ad initpm and lacks mammalian-type shelf
reorientation) and mammal. Previous studies have
shown that FU induces cleft palate in both birds and
mammals. Air sacs of quail eggs were injected with 100
pg FU in 0.1 m1 saline or 0.1 m1 saline only. Hamsters
were given intramuscular injection of 81 mg/kg FU in 1
m1 saline or 1 ml saline only. Total GAG synthesis was
measured by incorporation of 3~-glucosamineS. ulfated
and non-sulfated GAGs were identified by Alcian Blue
histochemistry combined with the use of GAGdegrading
enzymes. The results indicated that a
continuous synthesis of GAG at a steady rate was
associated with normal palate morphogenesis in both
quail and hamster. The amount of GAG synthesized in
hamster palate was four-fold higher than in quail palate.
In contrast to the developing hamster palate where the
predominant GAG was hyaluronate, the major GAGs
present during quail palate development were sulfated
and were concentrated on the nasal side. FU treatment
did not affect the rate of GAG synthesis in
the developing palate of quail. In contrast, FU
administration altered the rates of GAG synthesis. and
affected hyaluronate accumulation, during palate
morphogenesis in hamster. Following an analysis of data
of the present study and those from literature, it was
suggested that, in contrast to the proposed role of
hyaluronate in mammalian shelf reorientation, the
sulfated GAGs may contribute to shelf volume and may
be associated with tissue differentiation in both hamster
and quail. Also, critical differences in timings, rates, and
types of macromolecular synthesis between quail and
hamster may be related to the evolutionarily diverse
morphogenesis of palate seen in birds and mammals.
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