Publication: Distribution of components of basal lamina and dystrophin-dystroglycan complex in the rat pineal gland, differences from the rat pineal gland: differences from the
brain tissue and between the subdivisions of the gland
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Date
2010
Authors
Bagyura, Zsolt ; Pócsai, Károly ; Kálmán, Mihály
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The pineal gland is an evagination of the
brain tissue, a circumventricular neuroendocrine organ.
Our immunohistochemical study investigates basal
lamina components (laminin, agrin, perlecan,
fibronectin), their receptor, the dystrophin-dystroglycan
complex (ß-dystroglycan, dystrophin utrophin),
aquaporins (-4,-9) and cellular markers (S100,
neurofilament, GFAP, glutamine synthetase) in the adult
rat corpus pineale. The aim was to compare the
immunohistochemical features of the cerebral and pineal
vessels and their environment, and to compare their
features in the distal and proximal subdivisions of the
so-called ’superficial pineal gland’. In contrast to the
cerebral vessels, pineal vessels proved to be
immunonegative to α1-dystrobrevin, but immunoreactive
to laminin. An inner, dense, and an outer, loose
layer of laminin as two basal laminae were present. The
gap between them contained agrin and perlecan. Basal
lamina components enmeshed the pinealocytes, too.
Components of dystrophin-dystroglycan complex were
also distributed along the vessels. Dystrophin, utrophin
and agrin gave a ’patchy’ distribution rather than a
continuous one. The vessels were interconnected by
wing-like structures, composed of basal laminacomponents:
a delicate network forming nests for cells.
Cells immunostained with glutamine synthetase, S100-
protein or neurofilament protein contacted the vessels, as
well as GFAP- or aquaporin-immunostained astrocytes.
Within the body a smaller, proximal, GFAP-and
aquaporin-containing subdivision, and a larger, distal,
GFAP-and aquaporin-free subdivision could be
distinguished. The vascular localization of agrin and
utrophin, as well as dystrophin, delineated vessels
unequally, preferring the proximal or distal end of the
body, respectively.
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