Publication: Efficient uptake of mannosylated proteins by a human Schwann cell line
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Date
2009
Authors
Baetas-da-Cruz, Wagner ; Alves, Lucinéia ; Guimarães, Erick V. ; Santos-Silva, Alessandra ; Pessolani, Maria Cristina V. ; Barbosa, Helene S. ; Corte-Rea, Suzana ; Cavalcante, Leny A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Complex carbohydrate structures are
essential molecules of infectious microbes and host cells,
and are involved in cell signaling associated with
inflammatory and immune responses. The uptake of
mannose-tailed glycans is usually carried out by
macrophages, dendritic cells (DCs), and other
professional phagocytes to trigger MHC class I- and
MHC class II-restricted antigen presentation, and to
promote T cell effector responses. Since Schwann cells
(SCs) have been proposed as immunocompetent cells,
we investigated whether a human cell line (ST88-14
cells) could bind mannosylated ligands in a specific
manner. The saturation of uptake of mannosylated
molecules by ST88-14 cells and the internalization and
distribution pathway of these ligands were tested by
cytometry and confocal plus electron microscopy,
respectively. This uptake showed a dose-dependent
increase, the saturation point being reached at high
concentrations of mannosyl residues/240mM mannose.
Merging of man/BSA-FITC and S100 labeling showed
their partial, but, significant colocalization.
Ultrastructural analysis of ST88-14 cells after incubation
with HRP-colloidal gold, without or with subsequent
chasing at 37°C, showed an initial location on the cell
surface and temperature- and time-dependent
internalization of the probe. Our findings suggest an
efficient mannosylated ligand uptake system through
putative lectin(s) that may be operational in
inflammatory and immune responses.
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