Publication: Age and experimental obstructive emphysema.
A morphometrical study on the rat
Authors
Escolar Castellón, J.de D. ; Gallego, B. ; Escolar, M.A. ; Miñana Amada, C. ; Roche, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Age, as a risk factor in the development of
experimental obstructive emphysema, is proposed as the
hypothesis of this study.
Ninety-two Wistar rats were organized into two age
groups: adult (16 weeks) and middle-aged (56 weeks).
Each age group was subdivided into three groups: a
control group, consisting of unmanipulated animals; a
«cannula» group consisting of animals into whose
trachea a cannula was implanted; and a «valve» group,
consisting of animals into whose trachea a valve had
been implanted. The survival was one month. A
histomorphometric study was performed on the lungs
and the results were compared statistically. 1-hroughout
the experiment the amount of food consuni~~byi each
animal and the variations in weight were monitored.
After sacrifice, the lungs were processed for light
microscopy. Thirteen histomorphometric variables were
quantified and subsequently systematized into three
groups: those which quantified the size of' Iie distal
airspace («=ea of the al; ;)!:ir seci .: .,: ,.. , ., . lar chordn
and «mean linear interct:l>i . . il~o.,civ hich quantified the
tissue («wall tliicknt. - % : : : ,sue density», «interna1
perimeter of eacii ::i\ :c>liii- \c.i,iioii),, <(interna1 alveolar
perimeter per fielcl>, ~ i i i ~.,;a lvcolar section/section
perimetern); and those which quantified the elastic fibre
(«elastic fibre area», «elastic fibre perimeter», «elastic
fibre area/elastic fibre perimeter», selastic fibre density»
and «elastic fibre density per tissue density»). The
results were compared statistically and the sensitivity,
specificity and misclassification indices were calculated,
as well as the attributable and relative risk.
From the results, it was observed that, in general, the
animals of the valve and cannula groups in both age
groups displayed a decrease in food intake and a body
weight loss. The middle-aged animals were the only
group which displayed significant differences in al1 the
morphometric variables except wall thickness, when the
cannula and valve groups were compared with the
control group. In both the cannula and valve groups, the
Offprint [equests to: Dr. Juan de Dios Escolar, Departamento de
Ciencias Morfológicas, Facultad de Medicina, 50009 Zaragoza, Spain
values of the variables which quantified the distal
airspace increased, while the values of the variables
which quantified the lung tissue and the elastic fibre
decreased. In the manipulated middle-aged group, the
attributable risk of developing emphysema was 56.66%
and the relative risk 5.55; in the group of manipulated
adult animals, the attributable risk was 23.55% and the
relative risk 1.66.
The results of this study lead us to propose that the
middle-aged rats with experimental airflow obstruction
displayed a greater risk of developing emphysema than
the adult rats which were subjected to the same
procedure.
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