Publication:
Fertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen activators during interplay between gametes

Simple item page
dc.contributor.advisorCoy Fuster, Pilar
dc.contributor.authorGrullón, LA
dc.contributor.authorMondéjar Corbalán, Irene
dc.contributor.authorGarcía Vázquez, Francisco Alberto
dc.contributor.authorRomar Andrés, Raquel
dc.contributor.authorCoy Fuster, Pilar
dc.contributor.departmentFisiología
dc.contributor.otherFacultades de la UMU
dc.date.accessioned2026-03-30T12:07:11Z
dc.date.available2026-03-30T12:07:11Z
dc.date.copyright©2012 American Society for Reproductive Medicine, Published by Elsevier Inc
dc.date.issued2012-02-02
dc.description.abstractObjective: To detect plasminogen and plasminogen activators (PA) in oviduct and oocytes and to clarify the role of the plasminogen/plasmin system on mammalian fertilization. Design: Experimental prospective study. Setting: Mammalian reproduction research laboratory. Animal(s): Oviducts and ovaries from porcine and bovine females were collected at slaughterhouse. A total of 52 oviducts and 2,292 oocytes were used. Boar and bull ejaculated spermatozoa were also used. Intervention(s): Plasminogen concentration in oviductal fluid (OF) through the cycle was measured. Immunolocalization of plasminogen and PAs in oocytes was carried out before and after fertilization. Porcine and bovine oocytes were in vitro fertilized, with plasminogen and plasmin added to the culture medium at different concentrations. Main Outcome Measure(s): Plasminogen concentration in OF. Plasminogen and PAs immunolocalization in oocytes. Penetration and monospermy rates, number of spermatozoa in the ooplasma and on the zona pellucida (ZP) after IVF. Result(s): Oviductal fluid contains about 92 mg/mL of plasminogen. The mature oocyte shows immunoreactivity toward plasminogen and toward PAs on its oolemma and ZP. After fertilization, plasminogen and PAs immunolabeling decreases in the oocyte, suggesting its conversion into plasmin. When exogenous plasminogen is added to the IVF medium, sperm entry into the oocyte is hampered, suggesting that the role of plasminogen activation during fertilization is to reduce the number of (or to select) penetrating spermatozoa. Conclusion(s): The plasminogen/plasmin system is activated during gamete interaction and regulates the sperm entry into the oocyte
dc.formatapplication/pdf
dc.format.extent12
dc.identifier.citationMondéjar I, Grullón LA, García-Vázquez FA, Romar R, Coy P. Fertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen activators during interplay between gametes. Fertil Steril. 2012 Feb;97(2):453-61. doi: 10.1016/j.fertnstert.2011.11.032. Epub 2011 Dec 16. PMID: 22177313.
dc.identifier.doi10.1016/j.fertnstert.2011.11.032
dc.identifier.eissn1556-5653
dc.identifier.urihttp://hdl.handle.net/10201/224322
dc.languageeng
dc.relationSin financiación externa a la Universidad
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0015028211028081?via%3Dihub
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectIVF
dc.subjectOocyte activation
dc.subjectSperm-zona pellucida binding
dc.subjectPolyspermy
dc.subjectPlasminogen
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleFertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen activators during interplay between gametes
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication4e445f63-9090-4642-8e8d-615e9db16d41
relation.isAuthorOfPublication24843fe8-fa96-4462-a6c9-b3a817588cbf
relation.isAuthorOfPublication2b98ccaf-e8a5-4630-8cb2-e82099a62d59
relation.isAuthorOfPublication22fb1ef1-cda7-4ef0-982d-19fef265cc90
relation.isAuthorOfPublication.latestForDiscovery4e445f63-9090-4642-8e8d-615e9db16d41
relation.isDirectorOfPublication22fb1ef1-cda7-4ef0-982d-19fef265cc90
relation.isDirectorOfPublication.latestForDiscovery22fb1ef1-cda7-4ef0-982d-19fef265cc90
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PIIS0015028211028081.pdf
Size:
944.35 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.37 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos