Publication: Fertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen activators during interplay between gametes
Authors
Grullón, LA ; Mondéjar Corbalán, Irene ; García Vázquez, Francisco Alberto ; Romar Andrés, Raquel ; Coy Fuster, Pilar
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Facultades de la UMU
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Coy Fuster, Pilar
Publisher
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DOI
10.1016/j.fertnstert.2011.11.032
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info:eu-repo/semantics/article
Description
Abstract
Objective: To detect plasminogen and plasminogen activators (PA) in oviduct and oocytes and to clarify the role of the plasminogen/plasmin system on
mammalian fertilization.
Design: Experimental prospective study.
Setting: Mammalian reproduction research laboratory.
Animal(s): Oviducts and ovaries from porcine and bovine females were collected at slaughterhouse. A total of 52 oviducts and 2,292 oocytes were used.
Boar and bull ejaculated spermatozoa were also used.
Intervention(s): Plasminogen concentration in oviductal fluid (OF) through the cycle was measured. Immunolocalization of plasminogen and PAs in
oocytes was carried out before and after fertilization. Porcine and bovine oocytes were in vitro fertilized, with plasminogen and plasmin added to the
culture medium at different concentrations.
Main Outcome Measure(s): Plasminogen concentration in OF. Plasminogen and PAs immunolocalization in oocytes. Penetration and monospermy
rates, number of spermatozoa in the ooplasma and on the zona pellucida (ZP) after IVF.
Result(s): Oviductal fluid contains about 92 mg/mL of plasminogen. The mature oocyte shows immunoreactivity toward plasminogen and toward PAs
on its oolemma and ZP. After fertilization, plasminogen and PAs immunolabeling decreases in the oocyte, suggesting its conversion into plasmin. When
exogenous plasminogen is added to the IVF medium, sperm entry into the oocyte is hampered, suggesting that the role of plasminogen activation during
fertilization is to reduce the number of (or to select) penetrating spermatozoa.
Conclusion(s): The plasminogen/plasmin system is activated during gamete interaction and regulates the sperm entry into the oocyte
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Citation
Mondéjar I, Grullón LA, García-Vázquez FA, Romar R, Coy P. Fertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen activators during interplay between gametes. Fertil Steril. 2012 Feb;97(2):453-61. doi: 10.1016/j.fertnstert.2011.11.032. Epub 2011 Dec 16. PMID: 22177313.
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