Publication: Visualization of bioavailable liposomal doxorubicin using a non-perturbing confocal imaging technique
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Date
2001
Authors
Krishna, R. ; Ghiu, G. ; Mayer, L.D.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Commonly employed tissue processing
techniques can significantly alter tissue drug distribution
patterns for liposomal encapsulated drugs by virtue of
drug leakage via loss of membrane integrity. We report
here a method that has been developed to determine the
fluorescence of bioavailable doxorubicin (DOX) in
tissues after administration of liposomal DOX
formulations. A non-perturbing confocal fluorescence
microscopy (CFM) technique with image processing
analysis was used with unprocessed fresh tissues. This
method takes advantage of the fact that considerable
quenching occurs when DOX is within liposomes,
leading to the selective visualization of the fluorescence
due to DOX released from liposomes. We demonstrate
that fresh tissue confocal imaging can be applied to
provide detailed drug distribution information with
improved accuracy and is a superior method for
analyzing tissue distribution of liposome entrapped
fluorescent agents
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Citation
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