Browsing by Subject "Liposomes"

Now showing 1 - 3 of 3
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    In vitro and in vivo efficacy evaluation of new self-assembling curcumin loaded nanohyaluronan-glycerosomes on wound restoring in health and diabetic rats
    (Elsevier, ) Gómez García, Francisco José; Ramírez Andreo, Antonio; Manconi, María; Manca, María Letizia; Matricardi, Pietro; Di Meo, Chiara; Fernàndez-Busquets, Xavier; Martínez Díaz, Francisco; Salmerón Martínez, Diego; López Jornet, Pía; Ciencias Sociosanitarias
    In this study the wound healing efficacy of new self-assembling curcumin loaded nanohyaluronan-glycerosomes has been tested in healthy and diabetic rats. Curcumin was loaded in nanohyaluronan-liposomes and -glycerosomes, special vesicles obtained mixing curcumin, hyaluronan nanohydrogel and soy lecithin. Curcumin loaded liposomes and glycerosomes were also prepared, characterized and tested as references. The physico-chemical (size and size distribution, surface charge and morphology), and technological (entrapment efficiency, stability over time and skin delivery) characteristics and biological performances (safety, ability to protect in vitro cells from oxidative damage) of prepared formulations were measured. Finally, they were daily applied to skin wounds of healthy or diabetic rats. The animal skin was excided and analysed at days 3, 7 and 14. The macroscopic observation underlined the higher ability of curcumin loaded nanohyaluronan-glycerosomes to improve wound healing in diabetic rats, already on day 3 up to day 14 (p < 0.05). Histopathological analysis confirmed an accelerated re-epithelization in healthy rats, while increased angiogenesis in diabetic ones. The superior therapeutic efficacy of curcumin loaded nanohyaluronan-glycerosomes compared to other formulations can be related to their enhanced ability to deliver higher curcumin concentrations at the wound site due to a synergistic effect of glycerol, hyaluronan nanohydrogel and lecithin.
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    Intertrigo-like eruption caused by pegylated liposomal doxorubicin (PLD)
    (Springer, 2009-07) Sánchez Henarejos, Pilar; Ros Martínez, Silverio; Marín Zafra, Gema R.; Alonso-Romero, José Luis; Navarrete Montoya, Agustín; Medicina
    Pegylated liposomal doxorubicin (PLD) is a drug whose use is increasingly common. It has been associated with a lower rate of haematologic and cardiac side effects than its nonencapsulated form. However, mucocutaneous toxicity is quite frequent and can be severe. Here we provide a case report of a patient who developed an intertrigolike eruption during treatment with PLD.
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    Open Access
    Visualization of bioavailable liposomal doxorubicin using a non-perturbing confocal imaging technique
    (Murcia : F. Hernández, 2001) Krishna, R.; Ghiu, G.; Mayer, L.D.
    Commonly employed tissue processing techniques can significantly alter tissue drug distribution patterns for liposomal encapsulated drugs by virtue of drug leakage via loss of membrane integrity. We report here a method that has been developed to determine the fluorescence of bioavailable doxorubicin (DOX) in tissues after administration of liposomal DOX formulations. A non-perturbing confocal fluorescence microscopy (CFM) technique with image processing analysis was used with unprocessed fresh tissues. This method takes advantage of the fact that considerable quenching occurs when DOX is within liposomes, leading to the selective visualization of the fluorescence due to DOX released from liposomes. We demonstrate that fresh tissue confocal imaging can be applied to provide detailed drug distribution information with improved accuracy and is a superior method for analyzing tissue distribution of liposome entrapped fluorescent agents