Publication: Morphological properties of human thyroid tumor cells in collagen gel culture and metastatic or invasive ability
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Date
1993
Authors
Nishida, S. ; Hosokawa, K. ; Kusunoki, T. ; Koezuka, M. ; Akai, F. ; Nakano, T. ; Funasaka, K. ; yoshioka, Y. ; Murata, K. ; Hashimoto, S. ; Tomura, T.T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Using normal human thyroid cells and tumor
cells, the reconstruction of various diseased cells in
collagen gel as well as the relationship between the
morphology of colonies in collagen-embedded culture
and the biological behavior (benignity, malignancy,
metastasis, and invasion) of the original tumors were
studied. In collagen gel culture, normal thyroid cells
reorganized follicle-like constructions, and follicular
adenoma cells showed in vivo-like constructions.
However, two different types of colonies were observed
in cultures of cells from papillary carcinomas. One was
the branching type with many outgrowths projecting to
three dimensions and the other was the spherical type
without any outgrowths. These spherical colonies were
observed in al1 cases of papillary carcinoma, but varied
from one case to another. Metastasis and invasion were
detected during pathological examination in cases with a
high ratio of spherical colonies. Our results indicate that
cells from highly metastatic and invasive thyroid cancer
form spherical colonies in the collagen gel culture, and
that this collagen culture is a useful method for studying
the heterogeneity of tumor cells as well as the metastasis
and invasive ability of tumor cells in vitro.
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