Publication: Trichoderma harzianum como biofungicida para el
biocontrol de Phytophthora capsici en plantas de pimiento (Capsicum annuum L.)
Authors
Ezziyyani, Mohammed ; Pérez Sánchez, María Consuelo ; Sid Ahmed, Ahmed ; Requena, María Emilia ; Candela Castillo, María Emilia
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Facultad de Biología
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Publisher
Murcia: Universidad de Murcia, Servicio de Publicaciones
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DOI
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info:eu-repo/semantics/article
Description
Abstract
En los ensayos de control biológico en pimiento ejercido por Trichoderma harzianum (T.h.) sobre Phytophthora capsici (P.c.), agente causal de la podredumbre de pimiento, se ha optimizado la producción de la biomasa del antagonista T.h. al comparar su desarrollo en tres diferentes medios y soportes de cultivo. El método de producción de la biomasa de T.h. en Agua-Avena-Vermiculita resultó ser el mas rentable por su rápido y abundante crecimiento, viabilidad y bajo coste para utilizarlo como inóculo del suelo, al compararlo con los crecidos en Czapek líquido y PDB. El test del antagonismo in vitro de P.c. frente a T.h. en medio PDA enriquecido con Laminarina-glucosa (3:1, v/v), mostró que T. h. aumenta los niveles de enzimas hidrolíticas B-1,3- glucanasa (lisis enzimática), ejerce una mayor competencia por espacio y nutrientes e interacciona directamente con el patógeno (micoparasitismo), todo lo cual juega un papel importante en la reducción y destrucción de la colonia del patógeno. El filtrado del medio PDB donde se había cultivado T.h. afectó al crecimiento de las radículas de las semillas in vitro. En ensayos in vivo las plantas crecidas a partir de semillas tratadas mostraron un peso seco superior a las testigo. En definitiva, el tratamiento con T.h. ha sido capaz de reducir hasta un 65% la «tristeza» causada por el patógeno P.c. en plantas de pimiento.
During experiments in pepper plants using Trichoderma harzianum (T.h.) to control Phytophthora capsici (P.c.), the causal agent of root rot in pepper, we have optimised biomass production of the antagonist by comparing its growth in three different culture media and supports. Compared with Czapek liquid and PDB, the most advantageous me dium was Water-Oats-Vermiculite, since this provided the most rapid and abundant growth, showed the highest degree of viability and it was cheap for use as a soil inoculum. In vitro antagonism tests involving P.c. against T.h. in PDA medium enriched with Laminarine: L-glucose (3:1, v/v) showed that T.h. increases hydrolytic enzymes levels (enzymatic lyses), exercises a greater competition for space and nutrients, and directly interacts with the pathogen (micro-parasitism), all of whi ch play an important part in reducing and destroying the pathogenic colonies. The filtrate of the PDB medium in which T.h. had been cultu red affected the root growth of seedlings in vitro. In vivo experiments the plants grown from treated seeds showed a greater dry weight than the control plants. Treatment with T.h. reduced the wilt caused by P.c. in pepper plants by up to 65%.
During experiments in pepper plants using Trichoderma harzianum (T.h.) to control Phytophthora capsici (P.c.), the causal agent of root rot in pepper, we have optimised biomass production of the antagonist by comparing its growth in three different culture media and supports. Compared with Czapek liquid and PDB, the most advantageous me dium was Water-Oats-Vermiculite, since this provided the most rapid and abundant growth, showed the highest degree of viability and it was cheap for use as a soil inoculum. In vitro antagonism tests involving P.c. against T.h. in PDA medium enriched with Laminarine: L-glucose (3:1, v/v) showed that T.h. increases hydrolytic enzymes levels (enzymatic lyses), exercises a greater competition for space and nutrients, and directly interacts with the pathogen (micro-parasitism), all of whi ch play an important part in reducing and destroying the pathogenic colonies. The filtrate of the PDB medium in which T.h. had been cultu red affected the root growth of seedlings in vitro. In vivo experiments the plants grown from treated seeds showed a greater dry weight than the control plants. Treatment with T.h. reduced the wilt caused by P.c. in pepper plants by up to 65%.
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Citation
Ezziyyani, M., Pérez Sánchez, C., Sid Ahmed, A., Requena, M. E., & Candela Castillo, M. E. (2004). TRICHODERMA HARZIANUM COMO BIOFUNGICIDA PARA EL BIOCONTROL DE PHYTOPHTHORA CAPSICI EN PLANTAS DE PIMIENTO (CAPSICUM ANNUUM L.). Anales De Biología, (26), 35–45.
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