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Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice

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dc.contributor.authorUno, K.es
dc.contributor.authorKuroki, M.es
dc.contributor.authorHayashi, H.
dc.contributor.authorUchida, H.
dc.contributor.authorOshima, K.
dc.date.accessioned2011-06-30T12:00:27Z
dc.date.available2011-06-30T12:00:27Z
dc.date.issued2005
dc.description.abstractThe purpose of this study is to investigate the expression of thrombospondin-1 (TSP-1), a multifunctional extracellular matrix protein, during reepithelialization in wounded corneas of vitamin Adeficient mice. Epithelial defects were created in the corneas of normal and Vitamin A-deficient mice with a microgrinder. Wounded corneas were stained with fluorescein and photographed for evaluation of reepithelialization. Histological examination and immunohistochemical analysis of TSP-1 expression were also performed on the specimens from wounded corneas. In vitamin A-deficient mice, re-epithelialization of the wounded corneal epithelium was significantly delayed compared with that in normal mice. TSP-1 was detectable neither in the unwounded corneal epithelium of normal mice nor in that of vitamin A-deficient mice. In normal mice, linear staining of TSP-1 was observed on the wounded corneal surface and stroma at 30 min and 8 h to 16 h, respectively, after abrasion, and this TSP-1 expression disappeared at 36 to 48 h, when reepithelialization was completed. In contrast, no TSP-1 staining was observed in the wounded corneas of vitamin A-deficient mice, except for the endothelial cells, throughout the wound healing process. Histological examination revealed a progressive increase in polymorphonuclear neutrophil infiltration in the stroma of the corneas of vitamin A-deficient mice during the healing process. These findings suggest that vitamin A may modulate the expression of TSP-1 in the corneas to accelerate the re-epithelialization of wounded corneas.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/22492
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectVitamin A deficiencyes
dc.subjectCorneaes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::617 - Cirugía. Ortopedia. Oftalmologíaes
dc.titleImpairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient micees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.20, nº 2 (2005)