Publication: Impairment of thrombospondin-1 expression during epithelial wound
healing in corneas of vitamin A-deficient mice
Authors
Uno, K. ; Kuroki, M. ; Hayashi, H. ; Uchida, H. ; Oshima, K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The purpose of this study is to investigate the
expression of thrombospondin-1 (TSP-1), a
multifunctional extracellular matrix protein, during reepithelialization
in wounded corneas of vitamin Adeficient
mice. Epithelial defects were created in the
corneas of normal and Vitamin A-deficient mice with a
microgrinder. Wounded corneas were stained with
fluorescein and photographed for evaluation of reepithelialization.
Histological examination and
immunohistochemical analysis of TSP-1 expression
were also performed on the specimens from wounded
corneas. In vitamin A-deficient mice, re-epithelialization
of the wounded corneal epithelium was significantly
delayed compared with that in normal mice. TSP-1 was
detectable neither in the unwounded corneal epithelium
of normal mice nor in that of vitamin A-deficient mice.
In normal mice, linear staining of TSP-1 was observed
on the wounded corneal surface and stroma at 30 min
and 8 h to 16 h, respectively, after abrasion, and this
TSP-1 expression disappeared at 36 to 48 h, when reepithelialization
was completed. In contrast, no TSP-1
staining was observed in the wounded corneas of
vitamin A-deficient mice, except for the endothelial
cells, throughout the wound healing process.
Histological examination revealed a progressive increase
in polymorphonuclear neutrophil infiltration in the stroma of the corneas of vitamin A-deficient mice during
the healing process. These findings suggest that vitamin
A may modulate the expression of TSP-1 in the corneas
to accelerate the re-epithelialization of wounded corneas.
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